LOCUS       40924_23454       11663 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB615 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11663)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 11663)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11663)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11663)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1196..1285
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    1307..1431
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1468..1498
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1552..2208
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2553..2855
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2899..3023)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3049..3301
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3335..3351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3359..3375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3383..3413)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3428..3449)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3485..3737)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3900..4448)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4500..4679)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5185..5209)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5730..6518
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6767..7355
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7541..7681)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8025..8219)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8288..9358)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9790..10416)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaaca tcttttaccc
     1201 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     1261 tccatatgac gttccagatt acgctgctca gtgcagcgct gttatcacaa gtttgtacaa
     1321 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     1381 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggcccct
     1441 cgaggggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
     1501 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
     1561 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1621 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1681 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1741 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     1801 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1861 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1921 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1981 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     2041 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     2101 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     2161 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga
     2221 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     2281 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
     2341 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     2401 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     2461 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     2521 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
     2581 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     2641 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
     2701 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     2761 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     2821 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     2881 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     2941 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     3001 tcagctttct tgtacaaagt ggtgataacc tagagctcga atttccccga tcgttcaaac
     3061 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     3121 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     3181 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     3241 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     3301 cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     3361 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3421 gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat ttctattaat
     3481 tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     3541 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     3601 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     3661 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     3721 caaatgtttg aacgatcggg gaaattcgag ctggggacgt ccccagctcg gtaccccctc
     3781 ctgggcacgg tcgtacgcga tccgccgcgg ttccacctca ccattgcggg tatgccagtt
     3841 gagttcgatg agttcccgta ccagctccaa ctcgggcgat gccacgcgca ctccgttcag
     3901 atctcggtga cgggcaggac cggacggggc ggtaccggca ggctgaagtc cagctgccag
     3961 aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat gccgcggggg
     4021 gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg
     4081 accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta gagcgtggag
     4141 cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc cgtccagtcg
     4201 taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc gccgtccacc
     4261 tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt ccactcctgc
     4321 ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg
     4381 gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct
     4441 gggctcatat cttattgccc cccgggatca gatctggatt gagagtgaat atgagactct
     4501 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     4561 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     4621 gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg ttgcggttct
     4681 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     4741 ccttaattct ccgctcatga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa
     4801 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
     4861 tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg
     4921 caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
     4981 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt
     5041 tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctgatccgtc gagattaata
     5101 gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     5161 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     5221 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     5281 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     5341 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     5401 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa
     5461 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     5521 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     5581 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     5641 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     5701 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     5761 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     5821 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     5881 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     5941 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     6001 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     6061 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     6121 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     6181 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     6241 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     6301 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     6361 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     6421 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     6481 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     6541 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     6601 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     6661 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     6721 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6781 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6841 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6901 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6961 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7021 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7081 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7141 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7201 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7261 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7321 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7381 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7441 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7501 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     7561 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     7621 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     7681 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7741 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7801 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     7861 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     7921 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     7981 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     8041 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     8101 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     8161 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     8221 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     8281 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     8341 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     8401 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     8461 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     8521 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     8581 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     8641 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     8701 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     8761 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     8821 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     8881 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     8941 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
     9001 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
     9061 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
     9121 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
     9181 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
     9241 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
     9301 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc
     9361 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
     9421 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
     9481 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
     9541 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
     9601 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
     9661 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
     9721 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
     9781 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
     9841 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
     9901 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
     9961 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
    10021 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
    10081 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
    10141 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
    10201 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
    10261 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
    10321 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
    10381 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
    10441 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
    10501 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
    10561 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
    10621 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
    10681 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
    10741 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
    10801 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
    10861 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
    10921 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
    10981 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
    11041 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
    11101 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
    11161 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
    11221 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
    11281 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
    11341 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
    11401 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
    11461 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
    11521 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
    11581 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
    11641 caaatggacg aacggataaa cct
//
